{"componentChunkName":"component---src-templates-page-js","path":"/notes/strawberry-dna-extraction/","result":{"data":{"markdownRemark":{"html":"<p><a href=\"/img/uploads/2012/09/P9182052.jpg\"><img class=\"aligncenter size-full wp-image-56\" title=\"P9182052\" src=\"/img/uploads/2012/09/P9182052.jpg\" alt=\"\"  /></a></p>\n<p>Our assignment this week in Understanding Genomes: extract DNA from strawberries. The “common strawberry” is an octoploid, meaning each cell contains eight copies of each chromosome. This makes it a prime candidate for experimentation. Here's the step-by-step process:</p>\n<p>Step 1: Gather the ingredients. They are:</p>\n<ul>\n<li>Strawberries</li>\n<li>1/4 tsp table salt</li>\n<li>1 tsp dish detergent</li>\n<li>Meat tenderizer</li>\n<li>Water</li>\n<li>Rubbing alcohol (91% isopropanol)</li>\n</ul>\n<p>And other needed equipment, including:</p>\n<ul>\n<li>Jar</li>\n<li>Funnel</li>\n<li>Coffee filter</li>\n<li>Sandwich bag</li>\n<li>Wooden skewer</li>\n</ul>\n<p><a href=\"/img/uploads/2012/09/P9182040.jpg\"><img class=\"aligncenter size-full wp-image-50\" title=\"P9182040\" src=\"/img/uploads/2012/09/P9182040.jpg\" alt=\"\" /></a></p>\n<p>Step 2: Add the strawberries, detergent, salt and meat tenderizer in the sandwich bag and knead for about 5 minutes. This “buffer” opens the cells of the strawberries, releasing the DNA. Add a little water and mix when finished.</p>\n<p><a href=\"/img/uploads/2012/09/P9182041.jpg\"><img class=\"aligncenter size-full wp-image-51\" title=\"P9182041\" src=\"/img/uploads/2012/09/P9182041.jpg\" alt=\"\" /></a></p>\n<p><a href=\"/img/uploads/2012/09/P9182042.jpg\"><img class=\"aligncenter size-full wp-image-52\" title=\"P9182042\" src=\"/img/uploads/2012/09/P9182042.jpg\" alt=\"\"  /></a></p>\n<p>Step 3: Pour through coffee filter into a jar.</p>\n<p><a href=\"/img/uploads/2012/09/P9182044.jpg\"><img class=\"aligncenter size-full wp-image-53\" title=\"P9182044\" src=\"/img/uploads/2012/09/P9182044.jpg\" alt=\"\"  /></a></p>\n<p><a href=\"/img/uploads/2012/09/P9182049.jpg\"><img class=\"aligncenter size-full wp-image-54\" title=\"P9182049\" src=\"/img/uploads/2012/09/P9182049.jpg\" alt=\"\" /></a></p>\n<p>Step 4: Slowly pour chilled rubbing alcohol down the side of the jar so that it floats above the strawberry mixture. Since DNA is insoluble in alcohol, it begins to clump together.</p>\n<p><a href=\"/img/uploads/2012/09/P9182050.jpg\"><img class=\"aligncenter size-full wp-image-55\" title=\"P9182050\" src=\"/img/uploads/2012/09/P9182050.jpg\" alt=\"\"  /></a></p>\n<p>Step 5: After a few minutes, collect the DNA with a skewer. Mine looked like snot!</p>\n<p><a href=\"/img/uploads/2012/09/P9182055.jpg\"><img class=\"aligncenter size-full wp-image-58\" title=\"P9182055\" src=\"/img/uploads/2012/09/P9182055.jpg\" alt=\"\"  /></a></p>","frontmatter":{"path":"/notes/strawberry-dna-extraction/","title":"Strawberry DNA extraction","date":"2012-09-18T16:55:03.000Z","categories":["Understanding Genomes","Experiments","ITP"],"churl":null,"uses":null}}},"pageContext":{"post":true,"prev":{"url":"/notes/atmosfear/","title":"Atmosfear"},"next":{"url":"/notes/processing-spirograph/","title":"Processing Spirograph"}}}}